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native dna loading dye  (New England Biolabs)


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    Structured Review

    New England Biolabs native dna loading dye
    Native Dna Loading Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purple+dna+loading+dye/Gel+Loading+Dye+Purple/pm42081286-177-1-19
    Average 98 stars, based on 661 article reviews
    native dna loading dye - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Binding Assay:

    Article Title: A new family of CRISPR ‐type V nucleases with C‐rich PAM recognition
    Article Snippet: .. Ten microliter binding reactions were mixed with 2 μl of 6× Purple DNA loading dye (No SDS; NEB), supplemented with 10 mM Mg 2+ , and loaded onto a 5% nondenaturing PAA gel in 0.5× TBE (Thermo Fisher Scientific) buffer, supplemented with 5 mM Mg 2+ . .. Electrophoresis was carried out in 0.5× TBE buffer, supplemented with 5 mM Mg 2+ at 4°C for 3 h. Concentrations of bound substrate were calculated by evaluating the intensity of bands using ImageJ software and converting the intensities according to the following equation: c bound = I bound I bound + I free × c 0 , where c bound is the concentration of bound substrate, I bound is the intensity of the shifted band at a given enzyme concentration, I free is the intensity of the free substrate band at a given RNP concentration, and c 0 is the initial substrate concentration (2 nM).

    Concentration Assay:

    Article Title: RNA-specific local translation is patterned by condensates for multinucleate cell growth.
    Article Snippet: Each 20 μl reaction contained 1× electrophoretic mobility shift assay (EMSA) buffer (20 mM HEPES pH 7.4, 50 μM EDTA, 5% v/v glycerol, 1 mM DTT, 5 mM MgCl2, 10 μg ml −1 BSA and 150 mM KCl), 1.25 mg ml−1 E.coli tRNA and 0.5 μl murine RNAse inhibitor (NEB M0314S). .. Then 2 μl of purple DNA loading dye (NEB, B7024S) and 5 μl of protein were diluted in the Whi3 protein storage buffer (150 mM KCl, 50 mM HEPES at pH 7.4, and 5 mM BME) to the desired final concentration. ..

    Article Title: RNA-specific local translation is patterned by condensates for multinucleate cell growth
    Article Snippet: Each 20 μl reaction contained 1× electrophoretic mobility shift assay (EMSA) buffer (20 mM HEPES pH 7.4, 50 μM EDTA, 5% v/v glycerol, 1 mM DTT, 5 mM MgCl 2 , 10 μg ml −1 BSA and 150 mM KCl), 1.25 mg ml −1 E.coli tRNA and 0.5 μl murine RNAse inhibitor (NEB M0314S). .. Then 2 μl of purple DNA loading dye (NEB, B7024S) and 5 μl of protein were diluted in the Whi3 protein storage buffer (150 mM KCl, 50 mM HEPES at pH 7.4, and 5 mM BME) to the desired final concentration. ..

    Acrylamide Gel Assay:

    Article Title: Genome-Wide Investigation of Transcription Factor Occupancy and Dynamics Using cFOOT-seq
    Article Snippet: To terminate the deamination reaction, samples were heated at 85°C for 15 min, followed by the addition of 0.3 μL UDG (NEB) and further incubation at 37°C for 30 min. Substrate cleavage was induced by adding 0.5 μL of 2M NaOH and incubating at 95°C for 10 min. .. The mixture was then cooled on ice, combined with 10 μL of formamide and 2 μL of 6× purple DNA loading dye (NEB), and analyzed by 15% 8M urea-denaturing acrylamide gel electrophoresis in 0.5× TBE buffer. .. The 6-FAM signal was detected and quantified using the ChemiDoc MP imaging system (Bio-Rad).

    Electrophoresis:

    Article Title: Genome-Wide Investigation of Transcription Factor Occupancy and Dynamics Using cFOOT-seq
    Article Snippet: To terminate the deamination reaction, samples were heated at 85°C for 15 min, followed by the addition of 0.3 μL UDG (NEB) and further incubation at 37°C for 30 min. Substrate cleavage was induced by adding 0.5 μL of 2M NaOH and incubating at 95°C for 10 min. .. The mixture was then cooled on ice, combined with 10 μL of formamide and 2 μL of 6× purple DNA loading dye (NEB), and analyzed by 15% 8M urea-denaturing acrylamide gel electrophoresis in 0.5× TBE buffer. .. The 6-FAM signal was detected and quantified using the ChemiDoc MP imaging system (Bio-Rad).



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    Image Search Results


    Journal: The Journal of Biological Chemistry

    Article Title: Sirtuin 6 is a histone delactylase

    doi: 10.1016/j.jbc.2025.110795

    Figure Lengend Snippet: Materials

    Article Snippet: 6× NEB purple DNA loading dye , New England Biolabs (NEB) , B7025.

    Techniques: Protease Inhibitor, Staining, Membrane